orcid_16x16 ORCHID iD 


Tau assemblies enter the cytosol of neurons in a cholesterol sensitive manner
Benjamin J. Tuck, Taxiarchis Katsinelos, Lauren V. C. Miller, Shi Cheng, Marina Vaysburd, Claire Knox, Lucy Tredgett, Leo C. James, William A. McEwan. Jun 2021

Learning to count: determining the stoichiometry of bio-molecular complexes using fluorescence microscopy and statistical modelling
Sophia F. Mersmann, Emma JohnsTracer Yong. Will A. McEwan, Leo C. JamesEdward A.K. CohenJoe Grove July 2020

Published manuscripts

32) Microglia become hypofunctional and release metalloproteases and tau seeds when phagocytosing live neurons with P301S tau aggregates.
Brelstaff JH, Mason M, Katsinelos T, McEwan WA, Ghetti B, Tolkovsky AM, Spillantini MG.
Sci Adv. 2021 Oct doi: 10.1126/sciadv.abg4980. 

31) Targeted protein degradation using intracellular antibodies and its application to neurodegenerative disease
Benn JA, Mukadam AS, McEwan WA
Sem Cell Dev Biol 2021 Oct doi: 10.1016/j.semcdb.2021.09.012

30) Identification of cis-acting determinants mediating the unconventional secretion of tau
Katsinelos T, McEwan WA, Jahn TR, Nickel W.
Scientific Reports 2021 Jun 11:12946

29) Tau assemblies do not behave like independently acting prion-like particles in mouse neural tissue.
Miller LVC, Mukadam AS, Durrant CS, Vaysburd MJ, Katsinelos T, Tuck BJ, Sanford S, Sheppard O, Knox C, Cheng S, James LC, Coleman MP, McEwan WA.
Acta Neuropathol Commun. 2021 Mar 12;9(1):41. doi: 10.1186/s40478-021-01141-6. 

28) Target-induced clustering activates Trim-Away of pathogens and proteins
Zeng J, Santos AF, Mukadam AS, Osswald M, Jacques DA, Dickson CF, McLaughlin SH, Johnson CM, Kiss L, Luptak J, Renner N, Vaysburd M, McEwan WA, Morais-de-Sá E, Clift D, James LC. 
Nat Struct Mol Biol. 2021 Feb 25. doi: 10.1038/s41594-021-00560-2.

27) Enhancing mitophagy as a therapeutic approach for neurodegenerative diseases
Aman Y, Ryan B, Torsetnes SB, Knapskog AB, Watne LO, McEwan WA, Fang EF.
Int Rev Neurobiol. 2020;155:169-202. doi: 10.1016/bs.irn.2020.02.008.

26) Disassembly of Tau fibrils by the human Hsp70 disaggregation machinery generates small seeding-competent species
Nachman E, Wentink AS, Madiona K, Bousset L, Katsinelos T,  Allinson K, Kampinga H, McEwan WA, Jahn TR, Melki R, Mogk A, Bukau B, Nussbaum-Krammer C
J Biol Chem 2020 May 28. doi: 10.1074/jbc.RA120.013478

25) The Role of Antibodies and Their Receptors in Protection Against Ordered Protein Assembly in Neurodegeneration
Katsinelos T, Tuck BJ, Mukadam AS, McEwan WA.
Frontiers in Immunology 2019 May 31. doi: 10.3389/fimmu.2019.01139

24) Acute and rapid degradation of endogenous proteins by Trim-Away.
Clift D, So C, McEwan WA, James LC, Schuh M.
Nat Protoc. 2018 Sep 24. doi: 10.1038/s41596-018-0028-3.

23) IP6 is an HIV pocket factor that prevents capsid collapse and promotes DNA synthesis
Mallery DL, Márquez CL, McEwan WA, Dickson C, Jacques DA, Anandapadamanaban M, Bichel K, Towers GJ, Saiardi A, Böcking T, James LC
eLife 2018 Jun 7 e35335

22) Measurement of Tau Filament Fragmentation Provides Insights into Prion-like Spreading.
Kundel F, Hong L, Falcon B, McEwan WA, Michaels TCT, Meisl G, Esteras N, Abramov AY, Knowles TJP, Goedert M, Klenerman D.
ACS Chem Neurosci. 2018 Apr 8. doi: 10.1021/acschemneuro.8b00094.

21) A Method for the Acute and Rapid Degradation of Endogenous Proteins
Clift D, McEwan WA, Labzin LI, Konieczny V, Mogessie B, James LC, Schuh M.
Cell 2017 Dec 171 1692-1706

20) Cytosolic Fc receptor TRIM21 inhibits seeded tau aggregation.
McEwan WA, Falcon B, Vaysburd M, Clift D, Oblak AL, Ghetti B, Goedert M, James LC.
Proc Natl Acad Sci U S A. 2017 Jan 114 574-579

19) Surveillance for Intracellular Antibody by Cytosolic Fc Receptor TRIM21.
McEwan WA.
Antibodies 2016 Nov 5 21-33

18) HIV-1 uses dynamic capsid pores to import nucleotides and fuel encapsidated DNA synthesis.
Jacques DJ, McEwan WA, Hilditch L, Price AJ, Towers GJ, James LC
Nature 2016 Aug 536 349-353

17) TRIM21 Promotes cGAS and RIG-I Sensing of Viral Genomes during Infection by Antibody-Opsonized Virus.
Watkinson RE, McEwan WA, Tam JC, Vaysburd M, James LC.
PLoS Path 2015 Oct 11 e1005253

16) TRIM21-Dependent Intracellular Antibody Neutralization of Virus Infection
McEwan WA, James LC. Prog in Mol Biol and Transl
Science 2015 Jan 129 167-87

15) Host Cofactors and Pharmacologic Ligands Share an Essential Interface in HIV-1 Capsid That Is Lost upon Disassembly
Price AJ, Jacques DA, McEwan WA, Fletcher AJ, Essig S, Chin JW, Halambage UD, Aiken C, James LC.
PLoS Pathog 2014 Oct 10 e1004459

14) Intracellular sensing of complement C3 activates cell autonomous immunity
Tam JC, Bidgood SR, McEwan WA, James LC.
Science 2014 Sep 345 1256-70

13) Translocalized IgA mediates neutralization and stimulates innate immunity inside infected cells
Bidgood SR, Tam JC, McEwan WA, Mallery DL, James LC.
Proc Natl Acad Sci U S A 2014 Sep 111 13463-8

12) Intracellular antibody immunity
Watkinson RE, McEwan WA, James LC.
J Clin Immunol 2014 Jul 1:S30-4

11) Intracellular antibody-bound pathogens stimulate immune signaling via the Fc receptor TRIM21
McEwan WA, Tam JCH, Watkinson RE, Bidgood SR, Mallery DL, James LC.
Nat Immunol 2013 Apr 14 327-36

10) AAA ATPase VCP is Essential for TRIM21-mediated Virus Neutralization
Hauler F, Mallery DL, McEwan WA, Bidgood SR, James LC. Proc Natl Acad Sci U S A 2012 Nov 109 19733-8

9) Regulation of Virus Neutralization and the Persistent Fraction by TRIM21
McEwan WA, Hauler F, Williams CR, Bidgood SR, Mallery DL, Crowther RA, James LC.
J Virol 2012 Aug 86 8482-91

8) Diverse HIV viruses are targeted by a conformationally dynamic antiviral
Caines MEC, Bichel K, Price AJ, McEwan WA, Towers GJ, Willett BJ, Freund SMV, James LC.
Nat Struc Mol Biol 2012 Mar 19 411-6

7) Intracellular antibody-mediated immunity and the role of TRIM21
McEwan WA, Mallery DL, Rhodes DA, Trowsdale J, James LC.
Bioessays 2011 Nov 33 803-9

6) Restriction of the felid lentiviruses by a synthetic feline TRIM5-CypA fusion
Dietrich I, McEwan WA, Hosie MJ, Willett BJ.
Vet Immunol Immunopathol 2011 Oct 143 235-42

5) Antibodies mediate intracellular immunity through tripartite motif-containing 21 (TRIM21)
Mallery DL, McEwan WA, Bidgood SR, Towers GJ, Johnson CM, James LC.
Proc Natl Acad Sci U S A 2010 Nov 107 19985-90

4) Potent lentiviral restriction by a synthetic feline TRIM5 cyclophilin A fusion
Dietrich I, Macintyre A, McMonagle E, Price AJ, James LC, McEwan WA, Hosie MJ, Willett BJ.
J Virol 2010 Sep 84 8980-5

3) Truncation of TRIM5 in the Feliformia explains the absence of retroviral restriction in cells of the domestic cat
McEwan WA, Schaller T, Ylinen LM, Hosie MJ, Towers GJ, Willett BJ.
J Virol 2009 Aug 83 8270-5.

2) Genetically divergent strains of feline immunodeficiency virus from the domestic cat (Felis catus) and the African lion (Panthera leo) share usage of CD134 and CXCR4 as entry receptors
McEwan WA, McMonagle EL, Logan N, Serra RC, Kat P, Vandewoude S, Hosie MJ, Willett BJ.
J Virol 2008 Nov 82 10953-8.

1) The lingering effects of an artificial blind spot
Morgan MJ, McEwan W, Solomon J.
PLoS One 2007 Feb 2 e256.

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